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Journal: Journal of Translational Autoimmunity
Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis
doi: 10.1016/j.jtauto.2025.100345
Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.
Article Snippet: The
Techniques: Indirect ELISA
Journal: Frontiers in Immunology
Article Title: MSC-derived microvesicles inhibit the progression of arthritis in a murine model of preclinical rheumatoid arthritis
doi: 10.3389/fimmu.2026.1723572
Figure Lengend Snippet: Increased Expression of Serum Pro-Inflammatory Factors in Pre-CIA Mice. (A) ELISA was employed to measure the serum levels of key inflammatory markers and autoantibodies in the saline, Pre-CIA, and CIA groups. (B) interleukin-1β (IL-1β), (C) tumor necrosis factor-α (TNF-α), (D) interleukin-10 (IL-10), (E) interleukin-6 (IL-6), (F) interleukin-17a (IL-17a), (G) C-reactive protein (CRP), (H) anti-cyclic citrullinated peptide (anti-CCP) antibodies, and (I) anti-collagen type II (anti-CII) antibodies. The Pre-CIA group exhibited significantly higher levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-17a, and CRP) compared to the saline group, but the levels of pro-inflammatory cytokines in the Pre-CIA group were lower than those in the CIA group. The anti-inflammatory cytokine IL-10 and autoantibody levels (anti-CCP and anti-CII) exhibited a similar trend, reflecting an intermediate inflammatory state in the Pre-CIA group. Data are expressed as mean ± SD. Statistical comparisons were conducted using one-way ANOVA followed by Tukey’s post-hoc test. Significance levels were defined as two-tailed p < 0.05, with *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and N.S. indicating not significant.
Article Snippet: The serum concentrations of tumor necrosis factor alpha (TNFα, Abcam #ab208348), interleukin-1 beta (IL-1β, Abcam #ab197742), interleukin-17A (IL-17A, Abcam #ab199081), interleukin-10 (IL-10, Abcam #ab255729), C-reactive protein (CRP, Abcam #ab222511), interleukin-6 (IL-6 Abcam #ab222503), anti-cyclic citrullinated peptide antibody (anti-CCP, CUSABIO #CSB-EQ027743MO) and
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Saline, Two Tailed Test